Abcam
Anti-Wnt11 antibody
ab31962
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We injected adipose-derived stem cells into mice to detect the wound healing effects and signaling cascade involved. Several markers are chosen including alpha smooth muscle, Cytokeratin 10, Ki67, and Wnt11. We chose Abcam antibodies since the company has a good reputation of good quality.
Western Blot
Hep G2
4 degrees celcius overnight
5% milk
Goat anti-rabbit secondary antibody
Room temperature 1 hour
ECL
Clear specific band observed
None
Works well
NA
Works as expected