Abcam
Anti-C4d antibody
ab36075
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We wanted to detect complement activation in nasal tissue. Therefore we investigated if C4d was deposited in nasal tissue.
Immunohistochemistry
Frozen human nasal sinus tissue imbedded in OCT, fixed in PIPES/formaldehyde buffer for 10 min
1/80, ON at 4 C
10 % Donkey serum , 0.1 % triton X 100 in PBS
Donkey anti rabbit 1/800, 1 h, RT
NA
Fluorescent microscopy
C4d could be detected at the epithelium of the nasal tissue
None
Great for frozen sections
Great antibody for the detection of deposited C4d in frozen sections.