Excellent antibody to detect phospho-Pak in human cells

July 09, 2015

Physiology
University of Tennessee HSC
Postdoctoral Researcher

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Company:

Abcam

Product Name:

Anti-PAK1 + PAK2 + PAK3 (phospho T423) antibody

Catalog Number:

ab2477

Info: View Product View Product Specs

Browse Similar Products: T423 Antibodies

Our experiment was planned to check the effect of Pak1 knockdown in VEGFA induced Pak1 phosphorylation in Human retinal endothelial cells. Antibody showed its excellent specificity towards pPak1.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Protein lysate from human retinal endothelial cells treated with or without VEGFA

Primary Incubation

1:2000 (in 5%BSA in TBST), overnight incubation, 4 degrees C

Blocking Agent

5% skimmed milk in TBST

Secondary Incubation

1:4000 (in 5% skimmed milk in TBST), 2h, RT

Tertiary Incubation

NA

Detection

ECL

Results Summary

Above antibody specifically detect Pak1/Pak2/Pak3.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

No background noise signal

The Bad

None

The Bottom Line

Good antibody to detect pPak in human cells

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