Affymetrix eBioscience
Streptavidin APC
17-4317-82
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Anti-S1PR1 biotinylated aptamers were used to stain white blood cells. Aptamers were first incubated with Streptavidin APC and then added to cells. Streptavidin APC didn't show any significant binding to white blood cells without aptamers.
Labeling biotinylated aptamers
None
Streptavidin APC was incubated with biotinylated aptamers in DPBS buffer for 25 min before aptamers were used to stain white blood cells
Heat up aptamers to 95C and cool down before incubation with streptavidin
Streptavidin APC by itself didn't bind to white blood cells. S1PR1-aptamer-biotin-streptavidin complex staind the same population as anti S1PR1 Abs
Works well
Hard to estimate how much streptavidin to add per specific amount of aptamer
Binds to biotinylated aptamers / no non-specific binding to WBC
Product Name: Streptavidin APC 100 ugSupplier Page