Best Bioruptor for ChIP Assays

May 14, 2015

RAJIV GANDHI CENTRE FOR BIOTECHNOLOGY
MYCOBACTERIUM RESEARCH GROUP
SENIOR RESEARCH FELLOW

Overall

Performance

Ease-of-Optimization

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Company:

Diagenode

Product Name:

Bioruptor Standard

Catalog Number:

B01010002 (UCD 200 TO)

We use the Bioruptor for our Chromatin immuno-precipitation assays. It is essential for us to optimize the size of DNA to fit our requirements before antibody pull-down. This product allows us to do that.

Experimental Design and Results Summary

Application

ChIP experiments

Starting Material

Chromatin

Protocol Overview

Crosslink the samples with formaldehyde. After the crosslinking, this chromatin is chopped into a specific size of around 500bp using Diagenode' biorupter. As we use macrophage cell line we prefer to give 10 cycles (30 sec on and 30 sec off ). This sample is then taken and run on an agarose gel to confirm the size of chopped chromatin. After that, the pull down experiment will be carried out with specific antibodies.

Tips

Make sure the the sonicator tub is at 4 degrees celsius. If the machine is hot then stop for a while and then restart. Never give more than 10 cycles during a single stretch. Make sure the sample is maintained at lower temperature.

Results Summary

30 sec on and 30 sec off is considered one cycle. By giving 10 cycles we get a DNA of around 200-500BP in length.

Features Summary

Highly user friendly

Additional Notes

Easy to standardize the machine

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Summary

The Good

User friendly.

The Bad

No automatic temperature control. Becomes hot if we don't monitor the temperature every time.

The Bottom Line

Good machine that regularly works on ChIP experiments. Gives control over DNA chopping for ChIP experiments which is very important.

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