Thermo Scientific Pierce
SuperSignal West Pico Chemiluminescent Substrate
34077
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I use this chemiluminescent substrate to detect HRP conjugated secondary antibodies in order to detect proteins via Western blot. I used this chemiluminescent substrate because it is an easy two solution system that has consistently worked to detect HRP labeled secondary antibodies when I perform Western blots.
Western Blot
HRP labeled secondary antibodies
After incubated with HRP labeled secondary antibody, mix the two solutions in the kit 1:1 and then add to your membrane. Take pictures either on X-ray film or digitally.
Mix solutions well before adding to membrane
The Western blot image was taken digitally and shows a single dark band. This suggests the chemiluminescent substrate is being processed by HRP and producing detectable light.
Easy to use 2 solution system
None
Easy to use and works
This is a good chemiluminescent substrate source.
Product Name: SuperSignal West Pico Chemiluminescent SubstrateSupplier Page