Good source of Ethidium Bromide

University of Miami
Cell Biology
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Sigma-Aldrich

Product Name:

Ethidium Bromide

Catalog Number:

E-1510

I used this ethidium bromide solution to detect DNA in agarose gels. I used it to detect PCR, plasmid digest fragments, etc.

Experimental Design and Results Summary

Application

Staining DNA gels

Starting Material

Plasmid DNA

Protocol Overview

After running DNA on an agarose gel, post-stain with a solution of water and ethidium bromide for 15 minutes. Detect DNA bands with ultraviolet light (254 nm).

Tips

Dilute solution to 0.5 ug/ml

Results Summary

After post-staining the gel I saw bright DNA bands with very low background in the gel.

Additional Notes

None

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Summary

The Good

Easy to use

The Bad

None

The Bottom Line

This is a good source of ethidium bromide.

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