Good antibody to detect Actin

Cell Biology
University of Miami
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Anti-Actin Antibody

Catalog Number:

ab3280

Info: View Product View Product Specs

Browse Similar Products: Actin Antibodies

I regularly use this antibody to detect actin for loading controls for my Western blots. This antibody can be diluted 1:5000 and consistently works.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Primary Human Epithelial Cells

Primary Incubation

1:5000, Overnight, 4 celcius

Blocking Agent

5% Milk in 0.1% TBS-T

Secondary Incubation

HRP at 1:5000 for 1 hr at room temperature

Tertiary Incubation

NA

Detection

Chemilumenescence

Results Summary

1 band was detected by Western blot between 37 kDa and 50 kDa (actin = 42 kDa).

Additional Notes

None

Related Categories

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Summary

The Good

Detects Actin without need for optimisation

The Bad

None

The Bottom Line

This is a good antibody to detect actin in Western blots.

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