Very Good Anti-Human CD3 antibody

Dept of biomedical Sciences
TTUHSC El Paso
Post doc

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Tonbo Bioscience

Product Name:

CD3 PE Antibody

Catalog Number:

50-0039

Browse Similar Products: CD3 Flow Cytometry Antibodies

Human PBMC reconstituted NOD-scid IL2rγcnull mice (NSG mice), were sacrificed and single cell suspensions prepared from spleen were used for flow cytometric analysis. This antibody was used for detecting human CD3 expression on human CD45 gated cells isolated from these humanized mice. The results showed a clear CD3 positive population which confirms the specificity of this antibody.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Splenocytes from humanized mice

Primary Incubation

30 minutes

Blocking Agent

NA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometry on PE Channel

Results Summary

Flow cytometric analysis demonstrated a clear positive population for CD3 expression as compared to isotype antibody.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Very good clear signal and reproducibility

The Bad

Titration is required

The Bottom Line

Very good antibody for detecting human CD3 expression

Share your experience with other scientists. Write a Review! »

Join the discussion