Cell Signaling Technology
HA-Tag (6E2) Mouse mAb
2367S
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I used this anti-HA antibody to stain my N-terminal HA tagged NICD3 protein. NICD3 should primarily be in the nucleus and I see nice nuclear staining with the HA antibody. Overall, this is a good antibody to detect HA.
Immunofluorescence
Primary Human Epithelial Cells
1:500, Overnight, 4 Celcius
3% BSA
1:500, 1 hr, Goat anti-mouse 555
NA
Confocal Fluorescent Microscopy
I was able to easily detect my HA-tagged protein in the nucleus by confocal microscopy with this primary antibody. In addition, my protein needs to be nuclear and there is good co-localization with HA and nuclear dye.
None
Binds to HA with low background
This is a good anti-HA antibody and I would recommend this to anyone who needs to detect an HA tagged protein.