US Biological
FNC Coating Mix
BRFF AF-10
We used this coating mix for culturing and expanding human corneal endothelial cells (HCECs). As HCECs naturally adhere to Descement's membrane in the anterior chamber of the eye, we chose FNC to most closely mimic the composition of the membrane as well as provide necessary attachment proteins to create a healthy substrate.
HCEC Culture
Primary Tissue
Coating multiwell plates prior to cell seeding.
Manufacturer's recommended volume is more than one would think.
Good culture results, cell attachment optimal.
None
Performs as expected.
Cost.
Good product, does the job. Comes in 50mL bottles so they go fairly quickly and require a somewhat large volume to coat. Otherwise, they provide a great substrate for our cells and keep them attached.