Autophagy Western Blot Antibodies

Aging and Geriatric Research
University of Florida
Senior Laboratory Technician

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

Autophagy antibody sampler kit

Catalog Number:

CST# 4445

Browse Similar Products: Autophagy Western Blot Antibodies

Our goal is to identify and investigate the mechanisms of aging to better understand these mechanisms and eventually work towards the ability to extend human health span. This product was used to elucidate the effect of mechanical ventilation on oxidative stress and autophagy proteins on diaphragm of patients.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human diaphragm

Primary Incubation

1:1000 4C overnight

Blocking Agent

5% milk TBST

Secondary Incubation

1:10,000 room temperature - 1 hour

Tertiary Incubation

NA

Detection

Chemi substrate

Results Summary

Excellent detectable bands for human diaphragm samples, excellent positive control detection, low to very low background, no non-specific bands

Additional Notes

None

Related Categories

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Summary

The Good

Easy to use

The Bad

None

The Bottom Line

Very good antibody

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