Cell Signaling Technology
PIAS1 (D33A7) XP
3550
Browse Similar Products: PIAS1 Antibodies
I have tried several antibodies to detect PIAS1 (endogenous or in condition of over-expression) in cells. So far, this antibody is the best one for both Western blot and immunofluorescence analysis.
Western Blot
Different cell lines of different origins (human or mouse)
Overnight 4 degrees
Milk 5%/TBST
2 hours, room temperature, milk 1%/TBST
NA
HRP
Perfect clear and specific band. Image shows a lysate of HEK293T probed with the Ab anti-PIAS1 (D33A7) XP. Endogenous PIAS1 (lane 1) and the over-expressed (lane 2) level of 6xHistidine-tagged PIAS1 are shown. Lysis condition: Urea Lysis Buffer, gel 8%, transfer 3 hours 60 V.
None
It works perfectly.
I don't think there is a bad side.
The best antibody against PIAS1.