Anti-Mouse/Rat Foxp3 Alexa Fluor® 647

immunology
rigel inc.
scientist II

Overall

Quality of Results

Ease-of-Optimization

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Company:

eBioscience

Product Name:

Anti-Mouse/Rat Foxp3 Alexa Fluor® 647

Catalog Number:

51-5773-82

Browse Similar Products: FoxP3 Flow Cytometry Antibodies

I work in the field of immunology and sometime differentiate Treg from mouse Naive T cells in vitro. Anti-FoxP3 antibody, along with anti-CD4 and anti-CD25, allows me to identity iTreg from non-differentiate T cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Naive mouse T cells

Primary Incubation

Anti-Mouse/Rat Foxp3 Alexa Fluor® 647

Blocking Agent

Not necessary

Secondary Incubation

Not necessary (antibody is conjugated to Alexa Fluor® 647)

Tertiary Incubation

Not necessary

Detection

Flow Cytometry

Results Summary

This antibody work great. There is a good separation for Foxp3 population in my sample.

Additional Notes

Must follow eBioscience's staining protocol.

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Summary

The Good

It works great

The Bad

None

The Bottom Line

This product works very well

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