R&D Systems
Human Twist-1 Affinity Purified Polyclonal Ab, Sheep IgG
AF6230
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This was used for detection of Twist-1 in cancer cells using confocal microscopy. Twist-1 is an important player in EMT processes and it is essential to detect its presence in the nucleus of the cells.
Immunofluorescence
Colon cancer cells
1:200 in blocking buffer
0.05% FBS in PBS
Anti-sheep secondary antibody
Nucleus stain
Using Zeiss confocal microscope
Seems like at concentrations of 1:200, the staining is very good and at 1:100, there could be some interference. Had to adjust the secondary dilutions to get consistent staining.
None
Ease of standardization
None observed
Very good product to detect human twist-1