Abcam
Mouse anti-GAD65
ab26113
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Browse Similar Products: GAD65 Immunohistochemistry Antibodies
In these experiments, I wanted to investigate whether a novel receptor (for olfactory bulb tissue) was co-expressed with known subpopulations-the antibodies used target known histological markers for these subpopulations. To this end, I wanted to identify GAD65 location in GABAergic cells in fixed brain tissue. This antibody worked well at a low dilution and identified the processes of the GAD65-positive cells, but not the cell bodies.
Immunohistochemistry
PFA-fixed brain tissue slice
1:10,000 for 48 hours at 4 degrees C
1% normal horse serum in PBS (0.4% Triton-X 100)
1:1000 Cy3-conjugated horse anti-mouse for 2 hours at room temperature
NA
Cy3 fluorescence via fluorescent confocal microscopy
The cell processes stained, but the cell bodies did not. The dilution that gave the best pictures was much lower than the dilution recommended by the manufacturer. Worked better for IHC than for immunoblotting, but gave some response for both techniques.
None
Antibody works and gives good pictures.
Can be more expensive than other companies' equivalents.
Worked well for IHC, would not as readily recommend for immunoblot.