Best CX3CR1 antibody for immunohistochemistry

Department of Biological Sciences
Stanford University
Postdoc

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Company:

Aviva Systems Biology

Product Name:

CX3CR1 antibody - N-terminal region (OAAB12426) from Aviva Systems Biology

Catalog Number:

OAAB12426

The long-term goal of our lab is to understand the molecular and genetic mechanisms underlying brain development and disases. My work is to study the role of microglia in axon regenreation upon injury. CX3CR1 is a cell surface receptor which is highly expressed in microglia but not its precursor monocyte. Therefore, we use CX3CR1 as a marker to indicate microglia activation after spinal cord injury by immunohistochemistry in vivo. This product was used to detect whether lesion induce upregulation of CX3CR1; spinal cord was stained for Aviva Systems Biology CX3CR1 antibody.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Spinal cord

Primary Incubation

1:1000 at 4°C for 12 hours

Blocking Agent

3% BSA and 0.1% Triton 100 in TBS buffer

Secondary Incubation

1:2000 for 1 hour at 23°C

Tertiary Incubation

NA

Detection

Confocal imaging

Results Summary

The lesion side showed much higher expression levels of CX3CR1 compared to the control un-lesioned side of spinal cord.

Additional Notes

None

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Summary

The Good

Strong signal and specific

The Bad

None

The Bottom Line

This antibody is suitable for IHC of spinal cord tissue.

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