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PE/Cy7 anti-mouse CD25 Antibody
102016
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Mouse total T cells were collected and activated by anti-CD3/CD28 Ab (hamster derived) in vitro (pre-coated with goat anti-hamster antibody) for 3 days and stained with this antibody and CD4 antibody. During our experiments (including those run using this antibody), we used a number of different antibodies to look at the phenotype change of CD4 T cells. For example, if we want to look at CD62L subpopulation in CD4 Tcells, if CD62L is PE-conjugated, then we have to use CD4 FITC-conjugated, or PE-Cy7-conjugated (at least not PE channel). These are always dependent on the color set.
Flow Cytometry
Mouse spleen dervied T cells
1:200 dilution
PBS
N/A
FACS LSRII
After 3 days culture, almost all of the CD4 T cells express CD25.
None
Easy application
Can be used to detect activation status of CD4 T cells.