PE anti-mouse CD8a Antibody for CD8 staining

February 05, 2015

Cancer Biology
Scripps research center in FL
Research Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PE anti-mouse CD8a Antibody

Catalog Number:

100708

Mouse total T cells were collected and activated by anti-CD3/CD28 antibody (hamster derived) in vitro (pre-coated with goat anti-hamster antibody) for 3 days and stained with this antibody.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse spleen dervied T cells

Primary Incubation

1:200 dilution

Blocking Agent

PBS

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

FACS LSRII

Results Summary

After 3 days culture, around 50% are CD8+ T cells.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Easy application

The Bad

None

The Bottom Line

Can be used to gate CD8 + T cell population.

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