Brilliant Violet 421™ anti-mouse CD196 (CCR6) Antibody for memory T cell

January 21, 2015

Cancer Biology
Scripps research center in FL
Research Associate

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Company:

BioLegend

Product Name:

Brilliant Violet 421™ anti-mouse CD196 (CCR6) Antibody

Catalog Number:

129818

We transfer wild type CD4 T cells to Rag-/- mice to establish a Colitis model and then look at the phenotype of these transferred CD4 T cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse spleen

Primary Incubation

1:200 dilution

Blocking Agent

PBS

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

FACS LSRII

Results Summary

Spleen cells were stained with this Ab. Expression level of CCR6 was gated in CD4+CD25- cells.

Additional Notes

None

Related Categories

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Summary

The Good

Easy application

The Bad

None

The Bottom Line

Can be used to look at the memory T cell population

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