Novus Biologicals Inc.
LC3B
NB600-1384
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LC3B antibody was used to investigate autosomal recessive genetic disorder and detection especially during autophagy. Interaction between target protein and LC3B was tested. Among the several LC3B antibodies tested during investigation, one (NB600-1384) reviewed here produced best result for Western blot assay.
Western Blot
IB3-1, immortalized human bronchial epithelial cell line from cystic fibrosis patient
1:1000 dilution overnight at 4 degree in blocking reagent
3% nonfat dry milk (BioRad) in 0.1% tween20, PBS
1:5000 dilution of anti-mouse HRP for 1 hour at room temperature in blocking reagent
NA
Clarity Western ECL (BioRad), film
Antibody works good for detecting LC3B in Western blot assay with minor non-specific bands.
None
Specificity for detection
High cost
Very good antibody for detecting LC3B bands. Cost is pretty high for a small amount, but it only needs a small amount of antibody to detect LC3B