eBioscience
Anti-Human/Mouse CD49f (Integrin alpha 6) PE-Cyanine7
25-0495-82
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This antibody was used in a study that investigated effects of different EGFR Ligands on Human Mammary Epithelial Cell Differentiation. It is published in PLoS One in 2013 (Mukhopadhyay et al., Distinct Effects of EGFR Ligands on Human Mammary Epithelial Cell Differentiation. PLoS One. 4;8(10):e75907. PubMed PMID:24124521;). Many antibodies were tested but the antibody reviewed here gave the best result in FACS staining of Cd49f on the surface of live cells.
Flow Cytometry
Human Mammary Epithelial Cell
20 minutes on ice
1%FCS
NA
The antibody was conjugated with PEcy7 so FACS LSRII machine was used.
Very good
None
It is very specific if it is used at the right concentration.
Need to use an appropriate amount, otherwise there is a chance of getting nonspecific staining.
Overall the antibody is very good.