VEGF protein expression - Western blotting

November 24, 2014

Kli.Pha.-Tox
Charité - Universitatsmedizin Berlin, CCM
Ph.D.

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VEGF protein expression identified using VEGFA polyclonal antibody via Western blot.

Company:

Abnova Corporation

Product Name:

VEGFA polyclonal antibody

Catalog Number:

PAB12284

Info: View Product View Product Specs

Browse Similar Products: VEGFA Antibodies

VEGF protein expression was characterized via Western blotting using the VEGFA polyclonal antibody (1:1000) from Abnova.

Experimental Design and Results Summary

Applications

Western blot

Sample

Cell lysate of murine HL-1 cells

Primary Incubation

1:1000; overnight , 4°C , in TBS-T; 1%BSA; 0.02 Na3

Blocking Agent

5% low-fat milk solution

Secondary Incubation

1:5000, 1 h, room temperature, goat-anti-rabbit IgG – HRP conjugated

Tertiary Incubation

5% low-fat milk solution

Detection

ECL Plus Detection Reagents (Amersham)

Results Summary

Using the above mentioned antibody (1:1000), several bands were detected including VEGF-A dimer/monomer bands at approximately 43 kDa /23 kDa.

Additional Notes

NA

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VEGF protein expression identified using VEGFA polyclonal antibody via Western blot.

Summary

The Good

VEGF-A dimer/monomer bands at approximately 43 kDa /23 kDa.

The Bad

Several other (unknown) bands were detected.

The Bottom Line

Using the above mentioned antibody (1:1000) several bands were detected including VEGF-A dimer/monomer bands at approximately 43 kDa /23 kDa.  This antibody was okay for protein expression analyses via Western blotting in HL1 cells.

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