Phospho-Akt (Ser473) Mouse mAb from Cell Signaling Technology.
Cell Signaling Technology
12694S
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Phospho Akt/PKB protein expression was characterized via Western blot using thePhospho-Akt (Ser473) Mouse mAb (1:1000).
Western blot
Cell lysate of murine cardiomyocytes.
1:1000; overnight , 4°C , in TBS-T; 1%BSA; 0,02 Na3
5 % low-fat milk solution
1:5000, 1 h, room temperature, rabbit-anti-mouse IgG – HRP conjugated
5% low-fat milk solution
ECL Plus Detection Reagents (Amersham)
Using the above mentioned antibody (1:1000), one band was detected for phospho Akt at approximately 60 kDa.
NA
One band was detected for phospho Akt at approximately 60 kDa.
None
Using the above mentioned antibody (1:1000) one band was detected for phospho Akt at approximately 60 kDa. Antibody worked well in Western blot.