Beta-Actin protein expression analysis via Western blotting

October 16, 2014

Kli.Pha.-Tox
Charité - Universitatsmedizin Berlin, CCM
Ph.D.

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Indentification of beta-Actin expression using beta-Actin (N-21) antibody via Western blot.

Company:

Santa Cruz Biotechnology, Inc.

Product Name:

Beta-Actin (N-21) Antibody

Catalog Number:

sc-130656

Info: View Product View Product Specs

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Beta-Actin protein expression was characterized via Western blot using the anti-β-Actin (1:10000) from Santa Cruz

Experimental Design and Results Summary

Applications

Western Blot

Sample

Lysate of murine HL-1 cells

Primary Incubation

1:10000; overnight , 4°C , in TBS-T; 1%BSA; 0.02 Na3

Blocking Agent

5% low-fat milk solution.

Secondary Incubation

1:10000, 1 h, bei room temperature, goat-anti-rabbit IgG – HRP conjugated

Tertiary Incubation

5% low-fat milk solution

Detection

ECL Plus Detection Reagents (Amersham)

Results Summary

Beta-Actin was detected by this antibody in one distinct band (at 42 kDa) via Western blotting.

Additional Notes

NA

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Indentification of beta-Actin expression using beta-Actin (N-21) antibody via Western blot.

Summary

The Good

Beta-Actin was detected in one distinct band at 42 kDa, nice price

The Bad

NA

The Bottom Line

This antibody works well for Western blotting.

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