Very good antibody for detection of SHP2 by immunofluoresence

October 01, 2014

Cardiology
BIDMC
Postdoc

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review
SHP2 detected by immunofluorescence in mouse embryonic fibroblasts (MEF)

Company:

Santa Cruz

Product Name:

SH-PTP2 (C-18) Antibody

Catalog Number:

sc-280

Info: View Product

Browse Similar Products: SHPTP2 Antibodies 

The goal of this experiment was to determine the cellular localization of SHP2 in mouse embryonic fibroblasts (MEFs)

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse embryonic fibroblasts

Primary Incubation

1/200 dilution. 1 hour incubation at room temperature

Blocking Agent

5% normal goat serum

Secondary Incubation

1/400 dilution. 30 min at room temperature

Tertiary Incubation

None

Detection

Fluorescence microscope

Results Summary

This antibody works great to detect SHP2 in MEF cells by immunofluorescence. It allows detection of SHP2 in both the cytosol and the nucleus as expected when cells are at low density.

Additional Notes

Related Categories

Image Gallery

SHP2 detected by immunofluorescence in mouse embryonic fibroblasts (MEF)

Summary

The Good

Specific and cost-effective antibody

The Bad

None

The Bottom Line

I highly recommend this antibody to detect SHP2 in mouse cell culture. It is a quite cheap antibody, which gives a strong signal and can be used at a relatively high dilution.

Share your experience with other scientists. Write a Review! »

Join the discussion