Sigma
Mouse monoclonal anti-tubulin
T9026
Browse Similar Products: Anti-alpha tubulin Western blot Antibodies
Western blot detection usually requires the use of loading controls to interpret the results of experiments. Tubulin is ubiquitously expressed in the cell and is commonly used as a loading control in WBs. The T9026 is a great antibody that is easy to use and gives great blots.
Western Blot
HEK293 cell lysates in RIPA buffer
5% skim milk in TBST with anti-Tubulin 1/10000 - 1 hour at room temperature
5% skim milk in TBST- 10 minutes at room temperature
1/10000, room temperature for 1 hour in TBST 5% skim milk
NA
HRP- chemiluminescence
The tubulin antibody was blotted to demonstrate equal loading among four samples on one blot in HEK293T cells.
Very low antibody amount required for Western blot detection.
None
A great antibody overall and a must have for everyday Western Blotters!