Abcam
Anti-Hsp60 antibody (Abcam, ab46798).
ab46798
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Immunofluorescence in MEFs staining for Hsp60. Hsp60 was chosen as a mitochondrial marker to examine the mitochondrial network in MEFs.
Immunofluorescence
Mouse embryonic fibroblasts (MEFs)
1/6000 dilution of anti-Hsp60 in 5%BSA in PBS- 30 minute incubation
1% BSA in PBS - 30 minutes
Cy3-conjugated Goat anti-mouse, Jackson ImmunoResearch Laboratories- 30 minute incubation
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Carlo Zeiss Axiovert 200 microscope with a 63X oil fluorescence objective
Control MEFs stained for Hsp60 (orange/red) showing the mitochondrial network. Nuclei were visualized using DAPI staining.
None.
A great antibody for mitochondrial staining.
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Very potent antibody that's great for immunofluorescence!