CD48: Dim Fluorochrome

CSB
MGH
postdoc

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Biolegend

Product Name:

Alexa Fluor® 700 anti-mouse CD48 Antibody

Catalog Number:

103426

The aim of the research is to study bone marrow progenitor cells in cancer. In my experiment, I tested if a cytokine associated with tumor promotion alters cell counts. We selected CD48 Antibody from Biolegend specifically because CD48 is expressed in myeloid cells and can be found in bone marrow. Using this product I was able to differentiate myeloid subsets.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Bone marrow

Primary Incubation

1:300 30' in ice

Blocking Agent

FcBlock

Secondary Incubation

None

Tertiary Incubation

None

Detection

Fluorescence

Results Summary

No need to define the best working concentration. Pretty dim fluorochrome counterbalance a highly expressed marker. Some compensation required with APC, APC-Cy7 and Bv711.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Easy to use

The Bad

Not very bright

The Bottom Line

Useful for multicolor panels on limited sample amount

Share your experience with other scientists. Write a Review! »

Join the discussion