Great Transfer Apparatus

August 14, 2014

Overall

Performance

Ease-of-Optimization

What do these ratings mean?
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Jai Narain Vyas University, Jodhpur
Department of Biology
PhD student

Company:

Bio-Rad

Product Name:

Mini Trans-Blot Module

Catalog Number:

170-3935

I use this apparatus to transfer proteins from SDS-PAGE gels to PVDF membranes for immunoblotting.

We work on DNA damage pathways in our lab. This involves running a lot of gels for immunoblotting. The Biorad transfer apparatus is easy to use and one gets a very uniform and effecient transfer of proteins to PVDF membranes. That is why I chose to use this apparatus.

Experimental Design and Results Summary

Application

Transfer of proteins to PVDF membrane for mmunoblotting

Starting Material

HeLa cel lysates ran on a 4-12% gradient SDS-PAGE gel

Protocol Overview

HeLa cells were transfected, cell lysate was made and resolved on SDS-PAGE gels to perform immunoblotting

Tips

Check for the right orientation (black and red) and bubbles between the gel and membrane

Results Summary

The proteins transfer uniformly and the apparatus is easy to handle and assemble

Features Summary

Easy to use and efficient

Additional Notes

None

Related Categories

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Summary

The Good

Can be assembles easily. Possible to use for one or two gels

The Bad

Nothing to mention

The Bottom Line

I will always use this apparatus from BioRad for all my immunoblot transfers.

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