Children's Hospital of Eastern Ontario
Centogene Extan PMP22 Kit
Extan PMP22 Kit
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We are a clinical diagnostic lab. We used the extan kit to test its validity for use in the lab for full gene sequencing. The alternative to such a kit would be designing, optimizing and validating primers in house.
Sanger sequencing of the PMP22 gene for mutation detection
DNA
PCR, PCR clean up, Sequencing, PCR, Sequencing clean up, Sequence on 3730
Validated using a specific enzyme/kit and may require more optimization if you use something different
The kit was good, our results good. But we do not routinely use the reagents recommended by Centogene for this kit, and it needed optimization for it to work well with the reagents we have in house. It was not as simple as it appeared because more troubleshooting was needed.
None
Primers are validated and SNP free
May need more troubleshooting if you don't have the recommended kits in house
It's a fine, validated set of primers.
Product Name: EXTAN® PMP22 Primer Set KitSupplier Page