MRPS29 antibody

Overall

Quality of Results

Ease-of-Optimization

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neurobiology
Boston Childrens hospital
lab supervisor

Company:

BD Transduction Laboritories

Product Name:

DAP3 antibody

Catalog Number:

610662

Browse Similar Products: DAP3 Antibodies

Mitochondrial ribosomal protein S29 (MRPS29) is a mitochondrial pro-apoptotic protein also known as death associated protein 3 (DAP3). It is one of the components of small subunit of the mitochondrial ribosome. The antibody which was developed by BD Transduction Laboratories shows specific and good sensitivity targeting MRPS29.

Experimental Design and Results Summary

Applications

Western blot

Sample

Cell lysate (HeLa, K562, Jurkat, HEK293)

Primary Incubation

3% BSA in TBST, 4 degrees C for overnight incubation

Blocking Agent

5% skim milk

Secondary Incubation

3% BSA in TBST at room temperature for 2 hour incubation

Tertiary Incubation

N/A

Detection

Detected by ECL solution for HRP

Results Summary

It showed one band corresponded to MRPS29 with good sensitivity. As a loading control, mitochondiral protein HSP60 was used.

Additional Notes

None

Related Categories

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Summary

The Good

Good sensitivity with specificity

The Bad

None

The Bottom Line

It showed one band correspond to MRPS29 with good sensitivity.

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