Santa Cruz Biotechnology
Ankyrin G Antibody (P-20)
sc-31778
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Browse Similar Products: Ankyrin G Antibody Immunohistochemistry Antibodies
I work with protein trafficking in neurons. The described antibody was used to mark the axon initial segment in rat hippocampal neurons. This marker was chosen because it makes early recognition of the axon possible.
Immunohistochemistry
Rat hippocampal neurons
1:50 in PBS with 1 mM MgCl2, 0.1 mM CaCl2 and 0.2% gelatine
0.2% gelatine in PBS with 1 mM MgCl2, 0.1 mM CaCl2
Donkey anti-goat Alexa Fluor 647 1:1000 in PBS with 1 mM MgCl2, 0.1 mM CaCl2 and 0.2% gelatine
N/A
Zeiss 710 confocal microscope
Staining with this antibody enables detection of the axon initial segment, and it can therefore be used as a axonal marker in rat hippocampal neurons.
None
Specific identification of AIS
Very high concentration needed (1:50)
This antibody gives a specific detection of the axon initial segment.