EMD Millipore
Anti-BRCA2, Clone 5.23
05-666
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Browse Similar Products: Anti-BRCA2 Immunofluorescence Antibodies
The purpose of this experiment was to identify an antibody that was suitable for detection of BRCA2 in human cells by indirect immunofluorescence.
Immunofluorescence
Human hTERT-RPE1 cells
1:200 at room temperature for 60 minutes. Staining was performed in 1 x PBS and 0.1% Tween 20
Slide was pre blocked with 3% BSA in 1 x PBS and 0.1% Tween 20
1:100 dilution of Rhodamine Goat anti-mouse secondary diluted in 1 x PBS and 0.1% Tween-20 for 60 minutes at room temperature
No tertiary incubation was performed
Direct labeled goat anti-mouse antibodies
The antibody stains well and detecting a nuclear protein.
Cells were grown directly on slides and washed briefly with 1 x PBS before fixing and extracting for 10 minutes at room temperature in 4% formaldehyde, 1 x PBS and 0.1% Triton-X100
The signal is relatively strong with limited background
No drawbacks were evident with this antibody
Works for immunofluorescence. The antibody comes as 200ul which is sufficient for numerous staining experiments.