Active Motif
Anti-human MacroH2A raise in polyclonal mouse primary antibody
39593
Browse Similar Products: MacroH2A Immunofluorescence Antibodies
The human MacroH2A antibody from Active Motif does not work for immunofluorescence experiments in dilutions as low as 1:50, while antibodies from other companies do work at concentrations of 1:500. The IF experiment was put through several optimization experiments to detect MacroH2A in a variety of cell types including female hES/hiPS and 293 cells to visualize heterochromatin surrounding the X chromosome. It was also attempted to perform IF and FISH simultaneously, which works with other antibodies.
Immunofluorescence
293 cells fixed on slides, female hESC fixed on slides
1:75 - 1:500 tested, room temperature and 4C tested, protected from light and heat
IF blocking buffer: gelatin, BSA, RNase Inhibitor, PBS. Works in other IF applications
Goat anti-mouse Cy3-conjugated secondary antibody from AbCAM
N/A
Fluorescence microscopy
The antibody did not work for IF experiments in a variety of conditions tested. The MacroH2A antibody from Millipore works very well at concentrations of 1:500.
None
Cheap and small aliquots
Didn't work in the applications tested.
Do not use this antibody for IF experiments.