Fibroblast Activation: S100A4

Overall

Quality of Results

Ease-of-Optimization

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Cancer Research Program
Fundación IMIM
Lab manager

Company:

Millipore

Product Name:

Anti-FSP1/S100A4 Antibody

Catalog Number:

07-2274

S100A4 is a marker of fibroblast activation in vivo. Among many other processes, growth and maintenance of many tumors are regulated by fibroblasts of the stroma. Determination of the amount of fibroblast activation is an important parameter to get a real image of the status of the tumor.

Experimental Design and Results Summary

Applications

Western Blot

Sample

3T3-L1 mouse cell line

Primary Incubation

1/1000

Blocking Agent

Milk 5% in TBS-T

Secondary Incubation

Anti Rb HRP

Tertiary Incubation

Not needed

Detection

ECL

Results Summary

Unique band at 10 kDa

Additional Notes

15% acrylamide gel

Related Categories

Image Gallery

Summary

The Good

Specific signal. strong signal

The Bad

Any remarkable.

The Bottom Line

Good marker for fibroblast activation.

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