ECM Analysis: Fibronectin Detection

Overall

Quality of Results

Ease-of-Optimization

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Cancer Research Program
Fundación IMIM
Lab manager

Company:

Dako

Product Name:

Anti Fibronectin

Catalog Number:

A0245

Fibronectin1 (FN1) is one of the most abundant extra cellular proteins in ECM of the tumors. The quantity and direction of the FN1 matrix are important factors in tumor progression and growth. Checking these parameters in human samples or in animal induced tumors are a key factor in analysis of our daily research.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Cell extracts

Primary Incubation

1/2000

Blocking Agent

Milk 5% in TBS-T

Secondary Incubation

Anti Rb HRP

Tertiary Incubation

Not needed

Detection

ECL

Results Summary

Good detection with a small amount of protein extract.

Additional Notes

None

Related Categories

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Summary

The Good

Specific signal. Strong signal

The Bad

Any

The Bottom Line

Good detection of Fibronectin with 15 ug of total extracts

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