Pancreas Metabolism by IHC-Paraffin

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Quality of Results

Ease-of-Optimization

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Cancer Research Program
Fundación IMIM
Lab manager

Company:

SIGMA

Product Name:

Monoclonal Anti-Glucagon antibody produced in mouse

Catalog Number:

G2654

The main functions of pancreatic glucagon consist of gluconeogenesis, ketogenesis, lipolysis and glycogenolysis. Monoclonal anti-Glucagon antibody can be used as an analytical tool for quantification of the hormone.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mice pancreas

Primary Incubation

1/1000

Blocking Agent

1%FBS 1%BSA in TBS-T

Secondary Incubation

Donkey Anti ms

Tertiary Incubation

Not needed

Detection

Alexa 488

Results Summary

Glucagon is located in Pancreatic islet´s alpha cells as described. Citoplasmic signal is observed.

Additional Notes

Retrieval: citrate buffer (pH 6)

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Summary

The Good

Specificity.

The Bad

Any remarkable.

The Bottom Line

Good antobody. Easy to optimice even in paraffin sections of tissue.

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