Glut-4 Detection by IHC-Paraffin

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Cancer Research Program
Fundación IMIM
Lab manager

Company:

Millipore

Product Name:

Anti-Glucose Transporter GLUT-4 Antibody

Catalog Number:

CBL243

GLUT4 is a member of the solute carrier family 2 (facilitated glucose transporter) family and encodes a protein that functions as an insulin-regulated facilitative glucose transporter.We use it as a final marker of adypocite differentiation.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Pancreas asociated adipocytes

Primary Incubation

1/25

Blocking Agent

1%FBS 1%BSA in TBS-T

Secondary Incubation

Leyca Powervision

Tertiary Incubation

Not needed

Detection

DAB

Results Summary

Strong signal in membrane of the adipcytes. In any case should be expected a general staining of this marker.

Additional Notes

Retrieval: citrate buffer (pH 6)

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Summary

The Good

Specificity.

The Bad

Any remarkable.

The Bottom Line

Specific signal in expected cell types. Good election to work with.

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