EMD Millipore
Anti-BRCA1 (Ab-2) Mouse mAb (MS13)
OP93-100UG
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Human colon cancer cell line was stained with BRCA1 at 1/200 together with nuclear marker DAPI. The cells were PFA fixed, blocked in 3% normal goat serum and then incubated with the antibody for 12 hour at 4 degree. An Alexa conjugated goat polyclonal antibody was used as the secondary antibody. The image was acquired by confocal microscopy.
Immunofluorescence
Human colon cancer cell line
1:200 for 12hr at 4C
3% BSA plus 0.1% Triton-X 100 in TBS
Alexa Fluor 488 Goat Anti-Mouse IgG (H+L) 1:1000 for 1hr at 25C
Not used
Confocal imaging
BRCA1 antibody counter-stained with nuclear marker DAPI. The nuclear part of the human colon cancer cell was labeled, which consistent with its role as a component of the BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability.
N/A
Good signal with no background staining
None
This is a very good antibody to detect BRCA1 in cell line using immunofluorescence.