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FITC anti-human CD158a/h (KIR2DL1/S1) Antibody
339504
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Detecting KIRs (killer cell immunoglobulin-like receptors) on human natural killer cells is not always easy. We have found that some antibodies work better than others, despite the manufacturer's claims. This antibody gives a good signal and clear separation of positive vs. negative cells.
Flow Cytometry
Human natural killer cells
20uL of the antibody (neat) for 30 min on ice
Any generic human IgG
N/A
Flow cytometry (LSR-II from BD)
The results show KIR2DL1/S1 on the x-axis, plotted against KIR2DL3 on the y-axis. (gates applied: lymphocytes/singlets/CD3neg/CD56pos)
Good separation
Good antibody in a common flour (FITC)