Good Antibody at Affordable Price

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review
Department of Immunology/Division of Rheumatology
Mayo Clinic
Post doctoral fellow

Company:

BioLegend

Product Name:

Brilliant Violet 711™ anti-mouse/human CD11b Antibody

Catalog Number:

101241

Anti-mouse CD11b BV711 is a great antibody. Works good with cells from kidney. I selected this antibody for multicolor flow cytometry experiments to identify macrophages in kidney. Other markers we used are CD64 and F4/80. On BD Aria II sorter BV711 had very little or no compensation with colors on violet laser like BV421 or 650NC. The antibody concentration required is very less. For my experiments, I titrated 2ul antibody per 2*10^6 cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Kidney

Primary Incubation

45min

Blocking Agent

FCR block

Secondary Incubation

Not needed

Tertiary Incubation

Not needed

Detection

Violet laser 407A

Results Summary

I use this antibody to identify monocytes and macrophages in kidney in combination with markers like F4/80 and CD64

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Distinct populations; Can be used on human samples too.

The Bad

None

The Bottom Line

A good antibody. Worth using in multi parameter flowcytometry.

Share your experience with other scientists. Write a Review! »

Join the discussion