Antibody to N-Cadherin Protein Used in Epithelial to Mesenchymal Transition Research

November 04, 2013

Overall

Quality of Results

Ease-of-Optimization

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Cancer Research Program
Fundación IMIM
Lab manager

Company:

Abcam

Product Name:

Anti-N Cadherin Antibody

Catalog Number:

ab76011

Our lab is focused in epithelial to mesenchymal transition (EMT) research.We analize N-Cadh protein because it could suffer expression changes during EMT process due to transcriptional regulation of the CDH2 gen.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Mice embryo wt 15,5dpc in paraffin

Primary Incubation

Dilution 1/500. o/n, 4°C in TBS 1%BSA 1%FBS

Blocking Agent

TBS 1%BSA 1%FBS

Secondary Incubation

Leyca goat Powervision anti Rabbit. Undiluted. 25 min RT

Tertiary Incubation

Not needed

Detection

DAB

Results Summary

Antibody gives a strong specific signal in few seconds. Higher dilutions will work without problem.

Additional Notes

None

Related Categories

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Summary

The Good

Specifity and intensity of detection

The Bad

Not bad things to refer.

The Bottom Line

It's a perfect antibody to use in your IHC

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