Abcam
Anti-SIAT4A Antibody
ab21948
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Our lab is working on glycosyltransferases to find specific glycosyltransferase responsible for the synthesis of cancer associated glycans. ST3GAL1 is an important glycosyltransferase involved in the synthesis of cancer associated antigen known as STF (Sialyl Thomsen–Friedenreich). The immunofluorescence staining we performed with this antibody is one of our basic approaches to determine the expression of this enzyme in the Golgi apparatus. We select the Abcam antibody based on earlier experience in getting good results.
Immunofluorescence
Human cancer cells
1/200 in Blocking buffer for 1 hour at room temperature
3% BSA prepared in PBST (pH 7.2)
1/200 (in blocking buffer) with donkey anti-mouse-Alexa Fluor 488 (JIR Cat#715-546-150)
None
Confocal Micrscopy
We obtained reproducible and very high quality results with this antibody. The background staining which usually occurs in immunofluorescence staining is negligible with this antibody. The enzyme staining is observed in the Golgi area.
3% paraformaldehyde is used to fix the cells.
Highly sensitive and very specific
Overall, antibody gives very high quality results without any background.