Abcam
GCET1 mouse monoclonal antibody (RAM341)
ab68889
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GCET1 protein has been identified as playing an important role in germinal center B-cell physiology and as such, is deemed a marker for germinal center-like B-cells. I am using the GCET1 antibody to perform immunohistochemical (IHC) staining on diffuse large B-cell lymphoma sections, in order to assess GCET1 protein expression and immunophenotype the tumors as germinal center-like or activated B-cell like according to established IHC algorithms. All IHC is performed using the Ventana® BenchMark Automated Staining System.
IHC
4 micron sections cut from diffuse large B-cell lymphoma tumors
Mouse monoclonal (RAM341) antibody to GCET1. The antibody was used at a 1/80 dilution in Ventana Diluent. The protocol comprised a standard CC1 (Ventana) antigen retrieval , 32 minute antibody incubation, Ventana Amplification Kit (Amplifier A&B), 8 minute hematoxylin and 8 minute bluing reagent (Ventana).
No blocking agent is required using this antibody in conjunction with the Ventana iVIEW™ DAB Detection Kit.
Biotin conjugated secondary antibody (Ventana iVIEW™ DAB Detection Kit) comprising affinity purified biotinylated goat-anti-mouse IgG and IgM and goat-anti-rabbit IgG.
N/A
DAB (3, 3’-diaminobenzidine tetrahydrochloride)
The optimized staining conditions give a moderate strength cytoplasmic staining with minimal background.
None
Good value for money with specific staining.
Slight background can be a problem and the strength of staining can be weak (amplification required).
Good all round antibody with reliable results.