I express recombinant proteins with HA tag in mammalian cells and try to monitor condensate formation with osmotic stress. Since there are no good antibodies against my protein of interest, tagging with a small HA tag allows visualization of my protein with the help of immunofluorescence.
In my current work, I express recombinant proteins tagged with HA in mammalian cells to observe condensate formation under osmotic stress. Due to the absence of appropriate antibodies for my protein of interest, the use of a small HA tag allows for visualization via immunofluorescence.