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Tuesday, April 15, 2014
Multiplex qPCR commonly used for SNP detection, gene expression and other applications for which a large number of samples, or precious samples, need ...
Finding Cancer Biomarkers
Tuesday, April 01, 2014
Cancer researchers are constantly on the lookout for ways to predict disease outcome, determine which therapies are called for and track patient ...
Gene Expression Analysis
Thursday, February 27, 2014
Using next-generation DNA sequencing, cloud computing and in silico design tools, anyone can become expert in the gene expression of organisms outside...
Outsourcing miRNA Analysis
Tuesday, February 18, 2014
For many researchers, microRNA discovery and investigation are more a means to an end than the focus of their research. Or perhaps they simply are ...
Probing Protein-lncRNA Interactions
Tuesday, November 19, 2013
There is considerable research interest in the class of molecules called long noncoding RNAs (lncRNAs). Many have been identified, but few have had ...
Transcript Counting with smFISH
Tuesday, October 29, 2013
In its traditional incarnation, FISH is qualitative: Cells are either positive or negative for a given nucleic acid. But single-molecule quantitative ...
Software Tools for Gene Expression Analysis
Thursday, October 24, 2013
Gene-expression studies yield multitudes of data, and turning those data into biological insights requires software. A variety of options are ...
Choosing a qPCR Instrument
Thursday, September 26, 2013
qPCR is used for measuring RNA abundance, quantifying pathogen loads and validating microarray and sequencing data, among other applications. First, ...
RNA Prep from Problem Samples
Thursday, August 22, 2013
Expression analysis has become commonplace. First, though, you need to harvest the RNA. Here are tips for making the most of some difficult sample ...
Of Noncoding RNA and Epigenetics
Tuesday, July 23, 2013
Whether long, small or micro, noncoding RNAs are not translated into proteins, yet fulfill important regulatory roles in gene expression. Here we ...
Buyers' Guide to Thermal Cyclers
Monday, July 15, 2013
In the market for a PCR instrument? Here are four variables to consider.
High-res CNV Detection
Thursday, June 13, 2013
Traditionally, geneticists identified CNVs using fluorescence in situ hybridization (FISH) and early microarrays, but those methods had limited ...
Tuesday, May 21, 2013
Transcriptomics studies attempt to catalog and quantify the RNA content of a cell, tissue or organism. There essentially are three techniques for ...
Multiple Approaches to Single Cell Analysis
Thursday, May 09, 2013
Single cell analysis is a research area that is growing exponentially, having begun with more sensitive molecular biology tools. We have just seen the...
The Latest in qPCR Technologies
Tuesday, April 16, 2013
qPCR is a fluorescence-based, quantitative assay. As the reaction runs, its fluorescence intensity changes. A qPCR instrument monitors that ...
A Sequence of Expression
Tuesday, April 02, 2013
In short, our understanding genomics continues to rapidly evolve. While traditional, well-established techniques are still prevalent and powerful, ...
Very Strong Signal: Anti-CAF-1 IF
The purpose of this experiment was to identify an antibody that was suitable for detection of the chromatin assembly factor CAF-1 in human cells during S-phase ...
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