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Probing Protein-lncRNA Interactions
Tuesday, November 19, 2013
There is considerable research interest in the class of molecules called long noncoding RNAs (lncRNAs). Many have been identified, but few have had ...
Transcript Counting with smFISH
Tuesday, October 29, 2013
In its traditional incarnation, FISH is qualitative: Cells are either positive or negative for a given nucleic acid. But single-molecule quantitative ...
Software Tools for Gene Expression Analysis
Thursday, October 24, 2013
Gene-expression studies yield multitudes of data, and turning those data into biological insights requires software. A variety of options are ...
Choosing a qPCR Instrument
Thursday, September 26, 2013
qPCR is used for measuring RNA abundance, quantifying pathogen loads and validating microarray and sequencing data, among other applications. First, ...
RNA Prep from Problem Samples
Thursday, August 22, 2013
Expression analysis has become commonplace. First, though, you need to harvest the RNA. Here are tips for making the most of some difficult sample ...
Of Noncoding RNA and Epigenetics
Tuesday, July 23, 2013
Whether long, small or micro, noncoding RNAs are not translated into proteins, yet fulfill important regulatory roles in gene expression. Here we ...
Buyers' Guide to Thermal Cyclers
Monday, July 15, 2013
In the market for a PCR instrument? Here are four variables to consider.
High-res CNV Detection
Thursday, June 13, 2013
Traditionally, geneticists identified CNVs using fluorescence in situ hybridization (FISH) and early microarrays, but those methods had limited ...
Tuesday, May 21, 2013
Transcriptomics studies attempt to catalog and quantify the RNA content of a cell, tissue or organism. There essentially are three techniques for ...
Multiple Approaches to Single Cell Analysis
Thursday, May 09, 2013
Single cell analysis is a research area that is growing exponentially, having begun with more sensitive molecular biology tools. We have just seen the...
The Latest in qPCR Technologies
Tuesday, April 16, 2013
qPCR is a fluorescence-based, quantitative assay. As the reaction runs, its fluorescence intensity changes. A qPCR instrument monitors that ...
A Sequence of Expression
Tuesday, April 02, 2013
In short, our understanding genomics continues to rapidly evolve. While traditional, well-established techniques are still prevalent and powerful, ...
Mining Genetic Variation: The Latest SNP Analysis Tools
Wednesday, February 27, 2013
As their name suggests, single nucleotide polymorphisms (SNPs) are single-base sites of variation between peoples’ genomes, and they are, by far, the ...
State of the Art: miRNA Tools
Thursday, February 14, 2013
To start with, microRNAs (miRNAs) are short (20-¬¬ to 23-nucleotide) eukaryotic, noncoding regulatory RNAs that are enzymatically generated from ...
Transcriptomics: From Microarrays to RNA-Seq
Tuesday, January 08, 2013
RNA-Seq has largely solved many of the problems posed when using microarrays in transcriptome studies. By sequencing all the transcripts in a sample ...
TSG101 Antibody for Western Blot
Aviva's "TSG101 antibody -middle region" was tested on mouse brain protein lysates.
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