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RNA Prep from Problem Samples
Thursday, August 22, 2013
Expression analysis has become commonplace. First, though, you need to harvest the RNA. Here are tips for making the most of some difficult sample ...
Tools for Optimal RNA Prep: From Homogenization and Cell Lysis to Extraction and Purification
Wednesday, March 07, 2012
Extracting and purifying RNA from cells or tissues is no easy task, as anyone needing pure RNA for gene expression analysis will tell you. One of the ...
Isolation of RNA from Difficult Samples
Wednesday, September 22, 2010
RNA is already notoriously difficult to work with due to its instability, but RNA from “difficult samples” such as fixed tissue, blood, or single ...
Friday, September 04, 2009
There is a saying in many labs: “Garbage in, garbage out”. Since what goes “in” to so many assays, arrays, and reactions is RNA, you want to be sure ...
Staining of CD82 in Human Peripheral Blood Neutrophils
Human peripheral blood neutrophils were fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100 and blocked with 1% BSA for 30 minutes at 22°C. ...
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