Molecular cloning has come a long way since the first recombinant plasmids were constructed in the 1970s. Now a staple laboratory technique, cloning has undeniably enabled important developments in many biological applications, including protein expression, cell-based assays, mutagenesis, sequencing, gene editing, and more. Traditional cloning involves many sequential steps, such as DNA amplification, restriction digestion, ligation, and transformation, with additional DNA purification steps in between. Now, scientists can enjoy commercially prepared cloning and expression kits, which can offer the benefits of convenience, improved yield, greater genetic control, and more cloning options. For example, PCR cloning kits enable a simplified workflow for cloning directly from PCR products. To account for different expression systems, cloning and expression kits are also available that are specific to bacterial, mammalian, and yeast hosts. Cell-free protein expression kits are also available. Browse our catalog of cloning and expression kits from a diverse set of life science suppliers.
Countless positive outcomes have already emerged
Current methods and how they are being used.
The genomic DNA isolation kit (Blood/ Cultured Cell) was used for the isolation ...
Ideal kit for fast cloning of DNA inserts into a plasmid vector and in several ...
Our InvivO2 workstation is packed with innovative features that allow you to ...
A flexible medium pressure chromatography system that can be customized to meet ...