AccuSpot LC-MALDI Plate Spotter from Shimadzu

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ItemAccuSpot LC-MALDI Plate Spotter
CompanyShimadzu
Price Get Pricing Get Quote for this Product
Catalog NumberAccuSpot
Quantity1 System

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Shimadzu

7102 Riverwood Drive

Columbia, Maryland

United States

Phone: 1-800-477-1227
1-800-477-1227
1-410-381-1222

Company Profile

Website: https://www.ssi.shimadzu.com/

(1) Structural and Functional Characterization of Orcokinin B-like Neuropeptides in the Cuttlefish (Sepia officinalis)Marine DrugsAugust 4, 2022Maxime Endress et al.prepared in a diluent solution of 50% ACN containing 0.1% TFA. Twenty-second fractions were spotted by an AccuSpot spotter (Shimadzu) on stainless steel Opti-TOF™ 384 targets. Mass Spectrometry Analysis The MS experiments

(2) Physiological and Proteomic Insights Into Red and Blue Light-Mediated Enhancement of in vitro Growth in Scrophularia kakudensis—A Potential Medicinal PlantFrontiers in Plant ScienceJanuary 20, 2021Abinaya Manivannan, Prabhakaran Soundararajan, Yoo Gyeong Park, Byoung Ryong Jeongmatrix-assisted laser desorption/ionization–time-of-flight mass spectrometry (MALDI-TOF-MS) plate (AccuSpot, Shimadzu Ltd., Kyoto, Japan) and analyzed by a Voyager-DE STR mass spectrometer (Applied Biosystems,

(3) Tyrosine nitrations impaired intracellular trafficking of FSHR to the cell surface and FSH-induced Akt-FoxO3a signaling in human granulosa cellsAging (Albany NY)May 15, 2019Ge Zhou et al.50/50 acetonitrile/0.1% TFA) were automatically deposited onto MALDI target plates by the LC spotting system (AccuSpot, Shimadzu Biotech). These spotted samples were automatically analyzed by MALDITOF/TOF MS (MALDI-7090,

(4) Proteinaceous secretion of bioadhesive produced during crawling and settlement of Crassostrea gigas larvaeScientific ReportsDecember 1, 2018Valentin Foulon et al.prepared in a diluted solution of 50% ACN with 0.1% TFA. Twenty second fractions were spotted with an AccuSpot spotter (Shimadzu) on a stainless steel Opti-TOF™ 384 target. MS experiments were carried out on an

(5) Quantitative Proteomic Analysis Reveals That Arctigenin Alleviates Concanavalin A-Induced Hepatitis Through Suppressing Immune System and Regulating AutophagyFrontiers in ImmunologyAugust 16, 2018Qin Feng, Jingchun Yao, Ge Zhou, Wenkai Xia, Jingang Lyu, Xin Li, Tao Zhao, Guimin Zhang, Ningwei Zhao, Jie Yangpreviously (49). Eluates were automatically deposited onto MALDI target plates by the LC spotting system (AccuSpot; Shimadzu Biotech, Kyoto). These spotted samples were automatically analyzed by MALDI-TOF/TOF MS (MALDI-7090,

(6) Crustacean cardioactive peptides: Expression, localization, structure, and a possible involvement in regulation of egg-laying in the cuttlefish Sepia officinalisGeneral and Comparative EndocrinologyMay 1, 2018Maxime Endress et al.prepared in a diluent solution of 50% ACN containing 0.1% TFA. Twenty-second fractions were spotted by an AccuSpot spotter (Shimadzu) on stainless steel Opti-TOF™ 384 targets. 2.4.2.2 Mass spectrometry analysis MS experiments

(7) Glycosyltransferase MDR1 assembles a dividing ring for mitochondrial proliferation comprising polyglucan nanofilamentsProceedings of the National Academy of SciencesDecember 12, 2017Yamato Yoshida et al.and Presto FF-C18 column (1.0 × 150 mm; Imtakt) for separating the peptide mixtures in the samples, AccuSpot autospotter (Shimadzu) for spotting the separated samples onto the MALDI sample plates, and an AXIMA Performance

(8) Transglutaminase 2 is upregulated in primary hepatocellular carcinoma with early recurrence as determined by proteomic profilesInternational Journal of OncologyMay 1, 2017Hiromi Yamaguchi, Kazumichi Kuroda, Masahiko Sugitani, Tadatoshi Takayama, Kiyoshi Hasegawa, Mariko Esumitrifluoroacetic acid. The separated peptide samples were automatically spotted onto a µFocus MALDI plate using AccuSpot (Shimadzu Biotech). One microliter of a CHCA matrix solution (0.5 µg/µl) was applied and crystallized,

(9) EXTRACTION, ISOLATION AND STRUCTURAL ELUCIDATION OF FLAVONOID FROM CHROZOPHORA PLICATA LEAVES AND EVALUATION OF ITS ANTIOXIDATIVE POTENTIALSAsian Journal of Pharmaceutical and Clinical ResearchApril 1, 2017Kadiri Sunil Kumar, Avanapu Srinivasa Raoions can be separated and sorted. The mass spectrometer used for this purpose was SHIMADZU (SHIMADZU ACCUSPOT). HPTLC analysis was performed with CAMAG LINOMAT 5 instrument, and the images were observed at white

(10) Identification of the vascular endothelial growth factor signalling pathway by quantitative proteomic analysis of rat condylar cartilageFEBS Open BioJanuary 1, 2017Liting Jiang, Yinyin Xie, Li Wei, Qi Zhou, Xing Shen, Xinquan Jiang, Yiming Gaothen spotted onto target plates for MALDI‐TOF/TOF −4700 mass spectrometry (MS) measurements with the AccuSpot system (SHIMADZU). The MALDI‐TOF spectra were acquired in TOF/TOF mode using 10 laser shots per spectrum,

(11) Involvement of Brain-Enriched Guanylate Kinase-Associated Protein (BEGAIN) in Chronic Pain after Peripheral Nerve InjuryeneuroOctober 6, 2016Tayo Katano et al.were applied to a Prominence nano-liquid chromatography System (Shimadzu, Kyoto, Japan) coupled to an AccuSpot LC spotting system (Shimadzu). The mobile phases were solvent A [0.1% TFA in water/ACN (95:5, v/v)]

(12) Identification of citrullinated peptides in the synovial fluid of patients with rheumatoid arthritis using LC-MALDI-TOF/TOFClinical RheumatologySeptember 1, 2016Fei Wang et al.Kyoto, Japan). Finally, the desalted peptides were immediately applied to a prominence nano2D-HPLC and Accuspot™ system (Shimadzu Biotech, Kyoto, Japan). 2D-NanoLC fractionation A 5-μL aliquot of the desalted peptide/sample

(13) Characterization of Two Metal Binding Lipoproteins as Vaccine Candidates for Enterococcal InfectionsPLOS ONEAugust 31, 2015Felipe Romero-Saavedra, Diana Laverde, Aurélie Budin-Verneuil, Cécile Muller, Benoit Bernay, Abdellah Benachour, Axel Hartke, Johannes Huebnerprepared in a diluant solution of 50% ACN with 0.1% TFA. Twenty nine second fractions were spotted by an AccuSpot spotter (Shimadzu) on a stainless steel Opti-TOF 384 targets. MS experiments were performed on an AB

(14) Identification of Peptidoglycan-Associated Proteins as Vaccine Candidates for Enterococcal InfectionsPLoS ONENovember 4, 2014Felipe Romero-Saavedra, Diana Laverde, Dominique Wobser, Charlotte Michaux, Aurélie Budin-Verneuil, Benoit Bernay, Abdellah Benachour, Axel Hartke, Johannes Huebnerprepared in a diluent solution of 50% ACN with 0.1% TFA. Twenty nine second fractions were spotted by an AccuSpot spotter (Shimadzu) on a stainless steel Opti-TOF 384 targets. MS experiments were performed on an AB

(15) Salt Tolerance Enhancement of Liquid Chromatography-Matrix-Assisted Laser Desorption/Ionization-Mass Spectrometry Using Matrix Additive Methylenediphosphonic AcidMass Spectrom (Tokyo)January 1, 2014Yuki Ohta, Shinichi Iwamoto, Shin-ichirou Kawabata, Ritsuko Tanimura, Koichi Tanakaspotted onto the DHB-precoated plates with or without MDPNA using a dedicated spotter for LC-MALDIMS (AccuSpot; Shimadzu Corporation, Kyoto, Japan). The sample was analyzed using MALDI-QIT-TOFMS in positive-ion

(16) Transcriptome and Peptidome Characterisation of the Main Neuropeptides and Peptidic Hormones of a Euphausiid: The Ice Krill, Euphausia crystallorophiasPLoS ONEAugust 21, 2013Jean-Yves Toullec, Erwan Corre, Benoît Bernay, Michael A. S. Thorne, Kévin Cascella, Céline Ollivaux, Joël Henry, Melody S. Clarkprepared in a diluted solution of 50% CAN with 0.1% TFA. Fifteen second fractions were spotted by an AccuSpot spotter (Shimadzu) on a stainless steel Opti-TOFTM 384 target. MS experiments were carried out on an

(17) A Complex Set of Sex Pheromones Identified in the Cuttlefish Sepia officinalisPLoS ONEOctober 30, 2012Jérémy Enault, Céline Zatylny-Gaudin, Benoît Bernay, Benjamin Lefranc, Jérôme Leprince, Michèle Baudy-Floc'h, Joël Henryto 70% and leading to 360 15-second fractions mixed with the matrix and plated on the MALDI target (AccuSpot, Shimadzu). For each sample, two successive runs were performed to use two different matrixes: α-cyano-4-hydroxy

(18) Characterization of GnRH-related peptides from the Pacific oyster Crassostrea gigasPeptidesApril 1, 2012Laetitia Bigot, Céline Zatylny-Gaudin, Franck Rodet, Benoit Bernay, Pierre Boudry, Pascal Favrelin a diluting solution of 50% ACN with 0.1% TFA. 5 Fifteen second fractions were spotted by an Accuspot spotter (Shimadzu) on a stainless steel Opti-TOF™ 384 targets. MS experiments were realized on an AB

(19) Regulatory dendritic cells pulsed with carbonic anhydrase I protect mice from colitis induced by CD4+CD25- T cellsJournal of immunology (Baltimore, Md. : 1950)March 1, 2012Yamanishi H, Murakami H, Ikeda Y, Abe M, Kumagi T, Hiasa Y, Matsuura B, Onji M.and spotting on a mFOCUS MALDI plate (Shimadzu, Kyoto, Japan) were carried out automatically in a nano LC-AccuSpot apparatus (Shimadzu). Tandem time-of-flight mass spectrometry was performed on an AximaTOF2 mass spectrometer

(20) Purification of N-acetyllactosamine-binding Activity from the Porcine Sperm Membrane: Possible Involvement of an ADAM Complex in the Carbohydrate-binding Activity of SpermJournal of Reproduction and DevelopmentJanuary 1, 2012Etsuko MORI, Hiroyuki FUKUDA, Shinobu IMAJOH-OHMI, Tsuneatsu MORI, Seiichi TAKASAKIcontaining 0.1% TFA) and directly spotted onto a MALDI sample plate using a microfractionation system (AccuSpot, Shimadzu, Kyoto, Japan) for TOF-MS analysis. Peptide identification was carried out using a MALDI-TOF/TOF

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